Background

It is now possible to grow human brains cells (neurons and other cell types) in a dish using any other cell from the body (e.g. skin cells) by a process called induced pluripotent stem cells (iPSCs). Researchers are finding, however, that the surface of dishes used to grow brain cells—called the coating matrix—plays a crucial role in how well the cells grow, develop, and mature into specialized cell types, such as motor neurons. This study aimed to find the best coating matrix to ensure reliable growth and development of motor neurons , which are used to model diseases like ALS (Amyotrophic Lateral Sclerosis).

Research

We tested five different coating matrix conditions and identified a chemical called Polyethyleneimine was most effective for supporting the attachment and maturation of iPSC-derived motor neurons. Using these optimised conditions, we observed differences in the electrical properties of motor neurons generated from ALS patients compared to those from healthy individuals.

Potential Impact

Identifying the most suitable coating matrix will improve the reliability and consistency of lab-grown motor neurons. It paves the way for better disease models and could accelerate the discovery of treatments for neurological conditions like ALS.